Dramatically Enhanced Immunochromatographic Assay Using Cascade Signal Amplification for Ultrasensitive Detection of Escherichia coli O157:H7 in Milk

References

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ref
title
DOI
material type
comment
78Dramatically Enhanced Immunochromatographic Assay Using Cascade Signal Amplification for Ultrasensitive Detection of Escherichia coli O157:H7 in Milkhttps://doi.org/10.1021/acs.jafc.9b07076MetalAuNP-ICA platform

Materials

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ref
material
size
size err
size unit
size type
size comment
BET
b nanozyme
b 10n
b unit
specific act
sa 10n
sa unit
comment
78AuNP38nmTEMaverage diameter

Kinetics

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material
enzyme type
substrate
pH
T
km
km err
km 10n
km unit
vmax
vmax err
vmax 10n
vmax unit
kcat
kcat err
kcat 10n
kcat unit
kcat/km
kcat/km 10n
kcat/km unit
comment
78AuNP-AAPODTMB4RT0.27mM32.52-9M/s1145.1701/s78-3M-1 s-178
78AuNP-HQPODH2O24RT0.16mM20.16-9M/s724.6401/s78-3M-1 s-178
78AuNP-NH2OHPODTMB4RT0.58mM63.54-9M/s2218.3001/s78-3M-1 s-178
78AuNP-AAPODH2O24RT0.2mM26.24-9M/s924.0901/s78-3M-1 s-178
78AuNP-HQPODTMB4RT0.23mM25.95-9M/s913.5901/s78-3M-1 s-178
78AuNP-NH2OHPODH2O24RT0.41mM36.05-9M/s1269.3301/s78-3M-1 s-178

Applications

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material
application
target
method
linear range
linear ran unit
LOD
lod unit
recovery
comment
78AuNP-ICA platformDetection of Escherichia coli O157:H7 in MilkE. coliColor5-2.5*10^5CFU/mL12.5CFU/mL90.94