Ultrasmall CuS-BSA-Cu3 (PO4) 2 nanozyme for highly efficient colorimetric sensing of H2O2 and glucose in contact lens care solutions and human serum

References

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457Ultrasmall CuS-BSA-Cu3 (PO4) 2 nanozyme for highly efficient colorimetric sensing of H2O2 and glucose in contact lens care solutions and human serumhttps://doi.org/10.1016/j.aca.2020.02.064Compositeorganic-inorganic hybrid nanoscale materials, CuS-BSA-Cu3(PO4)2

Materials

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457CuS-BSA-Cu3(PO4)2100.21nmDLSTEM investigation of both CuS-BSA-Cu3(PO4)2 (Fig. 2) and CuS-BSA (Fig. S8) nanoparticles revealed that the nanomaterials consist of nanoparticles with an average diameter of 10 ± 0.21 nm and 9.68 ± 0.602 nm, respectively (the size was determined from 100 NPs using Image J software).
457CuS-BSA-Cu3(PO4)2121.834.5nmDLSthe average diameters of CuS-BSA and CuS-BSA-Cu3(PO4)2 are 134.5 ± 29.4 nm and 121.8 ± 34.5 nm

Kinetics

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457CuS-BSA-Cu3(PO4)2PODTMB5.5-4M8.2-8M/s457457
457CuS-BSA-Cu3(PO4)2PODH2O22.9-4M8.31-8M/s457457

Applications

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457CuS-BSA-Cu3(PO4)2detection of H2O2H2O2Color0–8μM22nM98.12-101.9%
457CuS-BSA-Cu3(PO4)2detection of H2O2H2O2Color0–8μM22nM98.12-101.9%Typically, using a standard addition method, H2O2 at 20, 50, and 100 nM is spiked into contact lens care solution obtained from a pharmacy. The H2O2 content present in lens care solution is determined from an already established calibration plot, generated at 654 nm using different H2O2 concentrations under the same assay mentioned above. Percentage recovery is assessed using Eq. (8), and the results are summarized in Table 3 (n = 3).