Nanozyme and aptamer-based immunosorbent assay for aflatoxin B1

References

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ref
title
DOI
material type
comment
424Nanozyme and aptamer-based immunosorbent assay for aflatoxin B1https://doi.org/10.1016/j.jhazmat.2020.123154Compositemesoporous SiO2/Au-Pt (m-SAP)/cDNA

Materials

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material
size
size err
size unit
size type
size comment
BET
b nanozyme
b 10n
b unit
specific act
sa 10n
sa unit
comment
424m-SAP/cDNA220.82nmTEMAs exhibited in Fig. 3C, the hydrodynamic diameters of m-SiO2 NP, MNP-aptamer and m-SAP/c-DNA are 201.09, 117.95, 220.82 nm, respectively. When MNP-aptamer and m-SAP/cDNA incubated and reacted, the m-SAP/MNP complex generated and showed a large size increase to 457.43 nm, implying the successful hybridization of aptamer and cDNA.

Kinetics

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material
enzyme type
substrate
pH
T
km
km err
km 10n
km unit
vmax
vmax err
vmax 10n
vmax unit
kcat
kcat err
kcat 10n
kcat unit
kcat/km
kcat/km 10n
kcat/km unit
comment
424m-SAP/cDNACATH2O20.016mM1.473-8M/s424
424m-SAP/cDNACATTMB0.058mM1.279-8M/s424

Applications

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material
application
target
method
linear range
linear ran unit
LOD
lod unit
recovery
comment
424m-SAP/cDNAdetection of aflatoxin B1 (AFB1)Aflatoxin B1Color0.01-1000ng/ml5pg/ml0.042000000000000003