Heparin-platinum nanozymes with enhanced oxidase-like activity for the colorimetric sensing of isoniazid

References

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171Heparin-platinum nanozymes with enhanced oxidase-like activity for the colorimetric sensing of isoniazidhttps://doi.org/10.1016/j.talanta.2019.120707Metalheparin sodium stabilized platinum nanoparticles (HS-PtNPs)

Materials

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171HS-PtNPs4.80.6nmTEMTEM image shows that the average diameter of HS-PtNPs was 4.8 ± 0.6 nm (Fig. 1C), and the high resolution TEM (HRTEM) image shows that HS-PtNPs possessed a 0.30 nm continuous lattice spacing2819.16U/g

Kinetics

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171HS-PtNPsOXDTMB0.01012mM171171

Applications

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171HS-PtNPsThese obvious advantages prompted us to explore the practical use of HS-PtNPs. The pyridine ring of isoniazid has strong reductive hydrazyl substitution, which can compete with TMB for the catalytic site of HS-PtNPs (Scheme 1). The introduction of isoniazid in HS-PtNPs-catalyzed oxidation process of TMB results in a lower efficiency and colorless reaction in TMB oxidation.isoniazidColor2.5-250μM1.7μM95%-103%