Enhancement of the peroxidase-like activity of aptamers modified gold nanoclusters by bacteria for colorimetric detection of Salmonella typhimurium

References

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DOI
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515Enhancement of the peroxidase-like activity of aptamers modified gold nanoclusters by bacteria for colorimetric detection of Salmonella typhimuriumhttps://doi.org/10.1016/j.talanta.2020.121476Metaldual aptamers modified bovine serum albumin stabilized-gold nanoclusters (aptamers@BSA-AuNCs)

Materials

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BET
b nanozyme
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specific act
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515aptamers@BSA-AuNCs1.770.51nmTEMThe average diameters of BSA-AuNCs and aptamers@BSA-AuNCs are 1.79 ± 0.52 nm and 1.77 ± 0.51 nm, respectively (Figs. S2B and S2D).

Kinetics

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515aptamers@BSA-AuNCsPODH2O25.53740.7mM14.47-8M/s515As shown in Table 1, with H2O2 as the substrate, the Km value of bacteria@aptamer1,2@BSA-AuNCs was similar to that of aptamer1,2@BSAAuNCs, demonstrating that the enhancement of the peroxidase-like activity of aptamer1,2@BSA-AuNCs did not originate from the increased affinity for H2O2. In comparison with aptamer1,2@BSA-AuNCs, the Vmax value of bacteria@aptamer1,2@BSA-AuNCs exhibited about 3 times improvement, which might be attributed to the enrichment of bacteria toward aptamer1,2@BSA-AuNCs and TMB, thus promoting the proximity of aptamer1,2@BSA-AuNCs to TMB and greatly increasing the local concentrations of the enzyme-mimetic nanoparticles and their substrate.
515aptamers@BSA-AuNCsPODTMB5.5370.3mM17.42-8M/s515515

Applications

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method
linear range
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515aptamers@BSA-AuNCsfor colorimetric detection of Salmonella typhimuriumSalmonella typhimuriumColor101-106cfu/mL1cfu/mL92.4% - 110%