ref | title | DOI | material type | comment |
---|---|---|---|---|
515 | Enhancement of the peroxidase-like activity of aptamers modified gold nanoclusters by bacteria for colorimetric detection of Salmonella typhimurium | https://doi.org/10.1016/j.talanta.2020.121476 | Metal | dual aptamers modified bovine serum albumin stabilized-gold nanoclusters (aptamers@BSA-AuNCs) |
ref | material | size | size err | size unit | size type | size comment | BET | b nanozyme | b 10n | b unit | specific act | sa 10n | sa unit | comment |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
515 | aptamers@BSA-AuNCs | 1.77 | 0.51 | nm | TEM | The average diameters of BSA-AuNCs and aptamers@BSA-AuNCs are 1.79 ± 0.52 nm and 1.77 ± 0.51 nm, respectively (Figs. S2B and S2D). |
ref | material | enzyme type | substrate | pH | T | km | km err | km 10n | km unit | vmax | vmax err | vmax 10n | vmax unit | kcat | kcat err | kcat 10n | kcat unit | kcat/km | kcat/km
10n | kcat/km unit | comment |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
515 | aptamers@BSA-AuNCs | POD | H2O2 | 5.5 | 37 | 40.7 | mM | 14.47 | -8 | M/s | 515 | As shown in Table 1, with H2O2 as the substrate, the Km value of bacteria@aptamer1,2@BSA-AuNCs was similar to that of aptamer1,2@BSAAuNCs, demonstrating that the enhancement of the peroxidase-like activity of aptamer1,2@BSA-AuNCs did not originate from the increased affinity for H2O2. In comparison with aptamer1,2@BSA-AuNCs, the Vmax value of bacteria@aptamer1,2@BSA-AuNCs exhibited about 3 times improvement, which might be attributed to the enrichment of bacteria toward aptamer1,2@BSA-AuNCs and TMB, thus promoting the proximity of aptamer1,2@BSA-AuNCs to TMB and greatly increasing the local concentrations of the enzyme-mimetic nanoparticles and their substrate. | |||||||||
515 | aptamers@BSA-AuNCs | POD | TMB | 5.5 | 37 | 0.3 | mM | 17.42 | -8 | M/s | 515 | 515 |
ref | material | application | target | method | linear range | linear ran unit | LOD | lod unit | recovery | comment |
---|---|---|---|---|---|---|---|---|---|---|
515 | aptamers@BSA-AuNCs | for colorimetric detection of Salmonella typhimurium | Salmonella typhimurium | Color | 101-106 | cfu/mL | 1 | cfu/mL | 92.4% - 110% |