Using bimetallic Au@ Pt nanozymes as a visual tag and as an enzyme mimic in enhanced sensitive lateral-flow immunoassays: Application for the detection of streptomycin

References

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DOI
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159Using bimetallic Au@ Pt nanozymes as a visual tag and as an enzyme mimic in enhanced sensitive lateral-flow immunoassays: Application for the detection of streptomycinhttps://doi.org/10.1016/j.aca.2020.06.009Multi-metalAu@Pt

Materials

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BET
b nanozyme
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159Au@Pt50nmTEMA typical TEM image (Fig. 3) showed that the Au@Pt nanozymes were relatively uniform in size and similar in structure, and the diameter of the nanoparticles was approximately 50 nm. As a uniform porous structure, Pt NPs formed a branched structure on the surface ofAu. The particle size ofthe Au corewas approximately 30e35 nm, and the average diameter of the Pt NPs was approximately 5e10 nm.

Kinetics

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159HRPPODTMB0.3296mM7.638-8M/s5.8941/s159
159Au@PtPODTMB2.431-3mM4.425-8M/s1.47531/s159
159HRPPODH2O21.468mM22.88-8M/s18.31/s159
159Au@PtPODH2O24.076-3mM6.013-8M/s2.00431/s159The apparent Km values for Au@Pt nanozyme were about 80e600 times smaller than that ofHRP, which indicated that the affinity between TMB/H2O2 and Au@Pt nanozyme was stronger than that between HRP and TMB/H2O2. Moreover, the Kcat values for Au@Pt nanozyme were about 80e340 times higher than that of HRP, which indicated that the catalytic efficiency of Au@Pt nanozyme was stronger than that of HRP

Applications

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159Au@PtAu@Pt nanozymes were introduced to develop a low-cost, rapid, visual and highly sensitive immunochromatographic assay for streptomycin detectionstreptomycinColor0.06ng/mlThe qualitative LOD was 0.1 ng mL−1 by the naked eye, and the quantitative LOD was 0.06 ng mL−1.
159Au@PtAu@Pt nanozymes were introduced to develop a low-cost, rapid, visual and highly sensitive immunochromatographic assay for streptomycin detectionstreptomycinColor0.06ng/ml