A label-free fluorescence biosensor based on a bifunctional MIL-101 (Fe) nanozyme for sensitive detection of choline and acetylcholine at nanomolar level

References

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168A label-free fluorescence biosensor based on a bifunctional MIL-101 (Fe) nanozyme for sensitive detection of choline and acetylcholine at nanomolar levelhttps://doi.org/10.1016/j.snb.2020.128021MOFMIL-101(Fe)

Materials

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168MIL-101(Fe)500nmSEMSEM images showed that MIL-101(Fe) had the well-defined octahedral morphology with an average diagonal length of approximately 500 nm2702.9

Kinetics

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168MIL-101(Fe)PODH2O24.0550.043mM5.138-7M/s168168
168MIL-101(Fe)PODTMB4.0550.585mM10.083-7M/s168

Applications

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168MIL-101(Fe)According to the enzyme cascade amplification strategy, the MIL-101(Fe) nanozyme in conjunction with AChE and ChOx provided a novel label-free fluorescent assay for detection of choline and ACh with high selectivity and sensitivity. Given this, this proposed sensing strategy was successfully utilized to detect the choline in milk and ACh in human plasma with desirable resultscholineFluor0.05-10μM20nM
168MIL-101(Fe)According to the enzyme cascade amplification strategy, the MIL-101(Fe) nanozyme in conjunction with AChE and ChOx provided a novel label-free fluorescent assay for detection of choline and ACh with high selectivity and sensitivity. Given this, this proposed sensing strategy was successfully utilized to detect the choline in milk and ACh in human plasma with desirable resultsAchFluor0.1-100μM8.9nM
168MIL-101(Fe)According to the enzyme cascade amplification strategy, the MIL-101(Fe) nanozyme in conjunction with AChE and ChOx provided a novel label-free fluorescent assay for detection of choline and ACh with high selectivity and sensitivity. Given this, this proposed sensing strategy was successfully utilized to detect the choline in milk and ACh in human plasma with desirable resultsH2O2Fluor0.1-130μM1.1nM