Aptamer-gold nanozyme based competitive lateral flow assay for rapid detection of CA125 in human serum

References

Loading, please wait
ref
title
DOI
material type
comment
362Aptamer-gold nanozyme based competitive lateral flow assay for rapid detection of CA125 in human serumhttps://doi.org/10.1016/j.bios.2020.112368MetalAptamer-gold nanozyme

Materials

Loading, please wait
ref
material
size
size err
size unit
size type
size comment
BET
b nanozyme
b 10n
b unit
specific act
sa 10n
sa unit
comment
362Aptamer-gold nanozyme21nmDLSthe average diameter of AuNPs to be ~10 nm .The absorption maxima of AuNPs after conjugation shifted from 521 nm to 530 nm (Fig. 2A) and particle size increases from 10 nm to 21 nm was observed (Fig. 2B and C).

Kinetics

Loading, please wait
ref
material
enzyme type
substrate
pH
T
km
km err
km 10n
km unit
vmax
vmax err
vmax 10n
vmax unit
kcat
kcat err
kcat 10n
kcat unit
kcat/km
kcat/km 10n
kcat/km unit
comment
362AuNPsPODTMB8.7719μM/min33641/min362

Applications

Loading, please wait
ref
material
application
target
method
linear range
linear ran unit
LOD
lod unit
recovery
comment
362Aptamer-gold nanozymeto develop an Aptamer-nanozyme lateral flow assay (ALFA)CA125 in human serumCL7.5-200U/mL5.21U/mL