A peroxidase-mimicking Zr-based MOF colorimetric sensing array to quantify and discriminate phosphorylated proteins

References

Loading, please wait
ref
title
DOI
material type
comment
137A peroxidase-mimicking Zr-based MOF colorimetric sensing array to quantify and discriminate phosphorylated proteinshttps://doi.org/10.1016/j.aca.2020.04.073MOFZr-based MOF

Materials

Loading, please wait
ref
material
size
size err
size unit
size type
size comment
BET
b nanozyme
b 10n
b unit
specific act
sa 10n
sa unit
comment
137Zr-MOF60nmSEMAs demonstrated by Fig. 1C, the synthesized Zr-MOF presents a uniform spherical morphology, and the diameter is ∼60 nm.217.5

Kinetics

Loading, please wait
ref
material
enzyme type
substrate
pH
T
km
km err
km 10n
km unit
vmax
vmax err
vmax 10n
vmax unit
kcat
kcat err
kcat 10n
kcat unit
kcat/km
kcat/km 10n
kcat/km unit
comment
137Zr-MOFPODTMB0.05mM137
137Zr-MOFPODH2O20.59mM137137

Applications

Loading, please wait
ref
material
application
target
method
linear range
linear ran unit
LOD
lod unit
recovery
comment
137Zr-MOFQuantification and discrimination of phosphorylated proteinsα-caseinColor0.17-5μg/mL0.16μg/mLFurther, the absorbance at 652 nm is linearly decreased with the increased levels of α-CS ranging from 0.17 to 5.0 μg/mL (Fig. 4B). The equation can be written as A = −0.0554[α-CS] (μg/mL) + 0.4119 (R2 = 0.996). The limit of detection (LOD) is calculated to be 0.16 μg/mL based on S/N = 3.
137Zr-MOFQuantification and discrimination of phosphorylated proteinsα-caseinColor0.17-5μg/mL0.16μg/mL