ref | title | DOI | material type | comment |
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178 | Biogenic synthesis of AuPd nanocluster as a peroxidase mimic and its application for colorimetric assay of acid phosphatase | https://doi.org/10.1016/j.colsurfa.2020.124444 | Multi-metal | AuPt nanoclusters (Au1Pd5 NCs) |
ref | material | size | size err | size unit | size type | size comment | BET | b nanozyme | b 10n | b unit | specific act | sa 10n | sa unit | comment |
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178 | Au 1 Pd 5 | 1.4 | nm | TEM | TEM was performed to investigate the size distribution of monometallic and bimetallic NCs. As shown in Fig. 2a and b, NADH-reduced Pd NCs with 24 h-incubation exhibit the mean size of 1.1 nm, while Au NPs show the average diameter of 8.1 nm. Remarkably, both Au1Pd5 and Au1Pd1 exist as highly dispersed NCs, showing the average size of 1.4 and 1.6 nm respectively (Fig. 2c and d). The mean size increases in the order of Pd < Au1Pd5 < Au1Pd1 < Au, which is consistent with the relative content of Au species. Hence, it is reasonable that NADH facilitates the rapid formation of ultrasmall NCs within a suitable range of [Na2PdCl4]/[HAuCl4]. Further, the atomic ratio in bimetallic NCs was determined by ICP-OES. The molar ratio of [Au]/[Pd] is 1.3 for Au1Pd1 and 0.25 for Au1Pd5, which is close to the corresponding theoretical ratio of two precursors in synthesis process. |
ref | material | enzyme type | substrate | pH | T | km | km err | km 10n | km unit | vmax | vmax err | vmax 10n | vmax unit | kcat | kcat err | kcat 10n | kcat unit | kcat/km | kcat/km
10n | kcat/km unit | comment |
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178 | Au1Pd5 NCs | POD | H2O2 | 160 | mM | 8.72 | -8 | M/s | 178 | ||||||||||||
178 | Au1Pd5 NCs | POD | TMB | 0.086 | mM | 6.48 | -8 | M/s | 178 |
ref | material | application | target | method | linear range | linear ran unit | LOD | lod unit | recovery | comment |
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178 | Au 1 Pd 5 | A colorimetric test is developed for quantitative determination of acid phosphatase. | Acid phosphatase (ACP) | Color | 1-14 | U/L | 0.53 | U/L | 102% 103% 99% | To validate the application of this method in human serum, spiked-recovery experiments were carried out with different concentration of ACP. To fit the linear range of the established calibration plot, commercial human serum was appropriately diluted before addition of ACP. The recovery rates are 102 % for 4 U/L, 103 % for 8 U/L and 99 % for 12 U/L ACP (listed in Table 1). The good recovery results guarantee the reliability of this method for estimating ACP activity in biological fluid. |
178 | Au 1 Pd 5 | A colorimetric test is developed for quantitative determination of acid phosphatase. | Acid phosphatase (ACP) | Color | 1-14 | U/L | 0.53 | U/L | 102% 103% 99% |