A Heparinase Sensor Based on a Ternary System of Hg2+–Heparin–Osmium Nanoparticles

References

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title
DOI
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222A Heparinase Sensor Based on a Ternary System of Hg2+–Heparin–Osmium Nanoparticleshttps://doi.org/10.1021/acs.analchem.9b05222CompositeHg2+–heparin–osmium nanoparticles (OsNPs)

Materials

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BET
b nanozyme
b 10n
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specific act
sa 10n
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222Hg2+/heparin–OsNPs80.97U/gTEM image of heparin–OsNPs. Inset: HRTEM image and the size distribution of heparin–OsNPs determined from the TEM image (from size distribution analysis of 50 random nanoparticles by Gaussian fitting).
222Hg2+/heparin–OsNPs81U/g

Kinetics

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pH
T
km
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222Hg2+/heparin–OsNPsOXDTMB0.051mM3.28-7M/s222Hg2+ 40μM
222Hg2+/heparin–OsNPsOXDTMB0.8mM8.45-8M/s222Hg2+ 0μM
222Hg2+/heparin–OsNPsOXDTMB0.176mM1.52-7M/s222222

Applications

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linear range
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LOD
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222Hg2+/heparin–OsNPsdetection of heparinase in human serum samplesheparinaseColor20-1000μg L-115μg L-1