Bioinspired Artificial “Clickase” for the Catalytic Click Immunoassay of Foodborne Pathogens

References

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title
DOI
material type
comment
1117Bioinspired Artificial “Clickase” for the Catalytic Click Immunoassay of Foodborne Pathogenshttps://doi.org/10.1021/acs.analchem.0c04732Compositecontaining abundant stable Cu(I) as an artificial “clickase” (namely, CCN) by using glutathione to stabilize Cu(I)

Materials

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material
size
size err
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size type
size comment
BET
b nanozyme
b 10n
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specific act
sa 10n
sa unit
comment
1117CCNTEMthe nanoparticles (NPs) of CCN clickases were nearly spherical, and had a diameter ranging from 30 to 80 nm, and the sizes of CCN clickases are suitable for use as labels for the immunoassay.8.8679

Kinetics

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material
enzyme type
substrate
pH
T
km
km err
km 10n
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vmax
vmax err
vmax 10n
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kcat
kcat err
kcat 10n
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kcat/km
kcat/km 10n
kcat/km unit
comment
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Applications

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material
application
target
method
linear range
linear ran unit
LOD
lod unit
recovery
comment
1117CCNCatalytic Click Immunoassay of Foodborne PathogensSalmonella enteritidisFluor100–1000000CFU/mL11CFU/mL