Ultrasensitive photoelectrochemical detection of microRNA on paper by combining a cascade nanozyme-engineered biocatalytic precipitation reaction and target-triggerable DNA motor

References

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203Ultrasensitive photoelectrochemical detection of microRNA on paper by combining a cascade nanozyme-engineered biocatalytic precipitation reaction and target-triggerable DNA motorhttps://doi.org/10.1021/acssensors.0c00632CompositeAu nanoparticles (NPs) modified by cyclodextrin (Au@CD)

Materials

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203Au nanoparticles (NPs) modified by cyclodextrin (Au@CD)20nmAs shown in Figure 2A, the Au@CD NPs showed good dispersity with a diameter around 20 nm.

Kinetics

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203Au@CD NPsPODH2O2100.01mM203203
203the mixture of Au@CD and CeO2PODH2O2800.34mM203As shown in Figure 4A–C, when H2O2 was used as the substrate, the Km value of Au@CD, CeO2, and the mixture of Au@CD and CeO2 were 100.01, 20.13, and 800.34 mM, respectively.
203CeO2PODH2O220.13mM203As shown in Figure 4A–C, when H2O2 was used as the substrate, the Km value of Au@CD, CeO2, and the mixture of Au@CD and CeO2 were 100.01, 20.13, and 800.34 mM, respectively.
203Au@CD NPsOXDDAB42mM203the GOx-like activity of Au@CD was first investigated by using DAB as the chromogenic substrates and an absorbance at 460 nm as the quantitative referenced wavelength. As shown in Figure S5, the Michaelis–Menten constant (Km) for the Au@CD NPs was 42 mM, which was distinctly higher than that of glucose oxidase (about 4.87 mM).

Applications

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203paper-based sensorMiRNA Detection.miRNA-141E-chem0.002-170pM0.6fM97.0–110.0%the recoveries and RSD were in the range of 97.0–110.0 and 1.31–13.64%, suggesting a gratifying analysis capability of the proposed sensor for miRNA-141 in complex clinical samples.
203paper-based sensorMiRNA Detection.miRNA-141E-chem0.002-170pM0.6fM97.0–110.0%