Handheld pH meter–assisted immunoassay for C-reactive protein using glucose oxidase–conjugated dendrimer loaded with platinum nanozymes

References

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781Handheld pH meter–assisted immunoassay for C-reactive protein using glucose oxidase–conjugated dendrimer loaded with platinum nanozymeshttps://doi.org/10.1007/s00604-020-04687-9Compositeglucose oxidase–conjugated dendrimer loaded with platinum nanozymes

Materials

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781PtDENs21.20.7nmDLSMoreover, the size of mAb-PtDEN-GOD (34.3 ± 1.6 nm) (Fig. 1f, bottom) was obviously more than that of PtDENs (21.2 ± 0.7 nm) (Fig. 1f, top) on the basis of DLS data
781mAb-PtDEN-GOD34.31.6nmDLSMoreover, the size of mAb-PtDEN-GOD (34.3 ± 1.6 nm) (Fig. 1f, bottom) was obviously more than that of PtDENs (21.2 ± 0.7 nm) (Fig. 1f, top) on the basis of DLS data
781PtNPs2.3nmTEMFigure 1c shows high-resolution transmission electron microscope (HRTEM) of the as-synthesized PtDENs. It is found that many PtNPs were distributed in the dendrimers, and the average size of nanoparticles was ~ 2.3 nm in diameter.
781mAb-PtDEN-GOD28nmTEMTo tackle this shortcoming, the as-prepared mAb-PtDEN-GOD conjugates were characterized by TEM after negative staining with sodium phosphotungstate (2.0 wt%, pH 7.3) (note: not good for negative staining of dendrimers). As seen from Fig. 1d, a layer of translucent structures was coated on the nearly spherical dendrimers, and the mean size was ~ 28 nm in diameter.

Kinetics

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Applications

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781mAb-PtDEN-GODassisted immunoassay for C-reactive proteinC-reactive protein (CRP)Unsure0.01-100ng/mL5.9pg/mLAs seen from Fig. 3c, pH variations could maintain ≥ 95% of the initial signal within 8 months. After storing for 11 months, the signal could also preserve more than 90%.