ref | title | DOI | material type | comment |
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781 | Handheld pH meter–assisted immunoassay for C-reactive protein using glucose oxidase–conjugated dendrimer loaded with platinum nanozymes | https://doi.org/10.1007/s00604-020-04687-9 | Composite | glucose oxidase–conjugated dendrimer loaded with platinum nanozymes |
ref | material | size | size err | size unit | size type | size comment | BET | b nanozyme | b 10n | b unit | specific act | sa 10n | sa unit | comment |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
781 | PtDENs | 21.2 | 0.7 | nm | DLS | Moreover, the size of mAb-PtDEN-GOD (34.3 ± 1.6 nm) (Fig. 1f, bottom) was obviously more than that of PtDENs (21.2 ± 0.7 nm) (Fig. 1f, top) on the basis of DLS data | ||||||||
781 | mAb-PtDEN-GOD | 34.3 | 1.6 | nm | DLS | Moreover, the size of mAb-PtDEN-GOD (34.3 ± 1.6 nm) (Fig. 1f, bottom) was obviously more than that of PtDENs (21.2 ± 0.7 nm) (Fig. 1f, top) on the basis of DLS data | ||||||||
781 | PtNPs | 2.3 | nm | TEM | Figure 1c shows high-resolution transmission electron microscope (HRTEM) of the as-synthesized PtDENs. It is found that many PtNPs were distributed in the dendrimers, and the average size of nanoparticles was ~ 2.3 nm in diameter. | |||||||||
781 | mAb-PtDEN-GOD | 28 | nm | TEM | To tackle this shortcoming, the as-prepared mAb-PtDEN-GOD conjugates were characterized by TEM after negative staining with sodium phosphotungstate (2.0 wt%, pH 7.3) (note: not good for negative staining of dendrimers). As seen from Fig. 1d, a layer of translucent structures was coated on the nearly spherical dendrimers, and the mean size was ~ 28 nm in diameter. |
ref | material | enzyme type | substrate | pH | T | km | km err | km 10n | km unit | vmax | vmax err | vmax 10n | vmax unit | kcat | kcat err | kcat 10n | kcat unit | kcat/km | kcat/km
10n | kcat/km unit | comment |
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ref | material | application | target | method | linear range | linear ran unit | LOD | lod unit | recovery | comment |
---|---|---|---|---|---|---|---|---|---|---|
781 | mAb-PtDEN-GOD | assisted immunoassay for C-reactive protein | C-reactive protein (CRP) | Unsure | 0.01-100 | ng/mL | 5.9 | pg/mL | As seen from Fig. 3c, pH variations could maintain ≥ 95% of the initial signal within 8 months. After storing for 11 months, the signal could also preserve more than 90%. |