Construction of a chiral artificial enzyme used for enantioselective catalysis in live cells

References

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ref
title
DOI
material type
comment
1418Construction of a chiral artificial enzyme used for enantioselective catalysis in live cellshttps://doi.org/10.1039/d0sc03082aCompositeyolk– shell Fe3O4@Poly(L-/D-Trp)

Materials

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material
size
size err
size unit
size type
size comment
BET
b nanozyme
b 10n
b unit
specific act
sa 10n
sa unit
comment
1418Polymer shell8.5nmDLSThickness
1418SiO2 shell25nmTEMThickness
1418Fe3O4 NPs200nmTEMaverage diameter

Kinetics

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material
enzyme type
substrate
pH
T
km
km err
km 10n
km unit
vmax
vmax err
vmax 10n
vmax unit
kcat
kcat err
kcat 10n
kcat unit
kcat/km
kcat/km 10n
kcat/km unit
comment
1418Fe3O4@Poly(L-Trp)PODL-tyrosinol6.040.82mM14.80.8531/s1418-6M-1 s-1
1418Fe3O4@Poly(L-Trp)PODD-tyrosinol14.080.96mM8.640.4331/s1418-6M-1 s-1
1418Fe3O4@Poly(D-Trp)PODL-tyrosinol22.732.46mM10.781.2731/s1418-6M-1 s-1
1418Fe3O4@Poly(D-Trp)PODD-tyrosinol6.490.86mM16.440.431/s1418-6M-1 s-1

Applications

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material
application
target
method
linear range
linear ran unit
LOD
lod unit
recovery
comment
1418Fe3O4@Poly(L-/D-Trp)Nanozymes were used to selectively label live yeast cells among yeast, S. aureus, E. coli and B. subtilis bacterial cells