Carbon quantum dots originated from chicken blood as peroxidase mimics for colorimetric detection of biothiols

References

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166Carbon quantum dots originated from chicken blood as peroxidase mimics for colorimetric detection of biothiolshttps://doi.org/10.1016/j.jphotochem.2020.112529CarbonCarbon quantum dots (CQDs) from chicken blood

Materials

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BET
b nanozyme
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166CB-CQDs1.5-3.6nmTEMThey exhibit a size range of 1.5–3.6 nm with an average diameter of about 2.4 nm, fitting well to the Gaussian function

Kinetics

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166CB-CQDsPODTMB4.5RT0.83mM5.13-8M/s166166
166CB-CQDsPODH2O24.5RT0.7mM4.09-8M/s166

Applications

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166CB-CQDsThe detection of biothiols was performed as follows: in a series of colorimetric tubes, 0.5 mL of TMB (20 mM), 0.5 mL of H2O2 (25 mM), and 0.1 mL of CB-CQDs were fully mixed in 3.8 mL of HAc-NaAc buffer at pH4.5. Then, various concentrations of biothiols standard solution (0.1 mL) were added into the above mixture. After they were well mixed and incubated at 40 °C for 25 min, the absorption spectra were recorded on a Unico 4802 ultraviolet-visible spectrophotometer at room temperature. The calibration curves for biothiols were established according to the decrease of absorbance defined as ΔA=A0﹣A, where A0 and A denote the absorbance at 652 nm without and with analyte, individually.cysteineColor0.5-20μM0.4μM95.9±2.7
166CB-CQDsThe detection of biothiols was performed as follows: in a series of colorimetric tubes, 0.5 mL of TMB (20 mM), 0.5 mL of H2O2 (25 mM), and 0.1 mL of CB-CQDs were fully mixed in 3.8 mL of HAc-NaAc buffer at pH4.5. Then, various concentrations of biothiols standard solution (0.1 mL) were added into the above mixture. After they were well mixed and incubated at 40 °C for 25 min, the absorption spectra were recorded on a Unico 4802 ultraviolet-visible spectrophotometer at room temperature. The calibration curves for biothiols were established according to the decrease of absorbance defined as ΔA=A0﹣A, where A0 and A denote the absorbance at 652 nm without and with analyte, individually.cysteineColor0.5-20μM0.4μM95.9±2.7105.7±2.0; 109.3±1.1; 99.7±4.3; 91.5±1.0; 98.2±2.3