Biomineralization-inspired copper-cystine nanoleaves capable of laccase-like catalysis for the colorimetric detection of epinephrine

References

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682Biomineralization-inspired copper-cystine nanoleaves capable of laccase-like catalysis for the colorimetric detection of epinephrinehttps://doi.org/10.1007/s11705-020-1940-yCompositeInspired by the pathological biomineralization behaviour of L-cystine, in this study, we constructed a laccase-like catalyst through the co-assembly of L-cystine with Cu ions. Structural analysis revealed that the formed catalytic Cu-cystine nanoleaves (Cu-Cys NLs) possess a Cu(I)-Cu(II) electron transfer system similar to that in natural laccase. Reaction kinetic studies demonstrated that the catalyst follows the typical Michaelis-Menten model.

Materials

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682Cu-Cys NLsnmTEMThe enlarged image shows that the NLs are approximately 16.2 nm thick (Fig. 1(b)), 450 nm long and 300 nm wide (Fig. 1(c)).

Kinetics

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682Cu-Cys NLshydrolase2,4-DP6.8250.1mM1.44μM/min682kcat/km unit: ×10-3 g -1 min-1 L

Applications

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682Cu-Cys NLsdetetion of epinephrineepinephrineColor9–455μM/L2.7μM/L100.1-108.3%