Nanozyme Database

Label-free determination of thyroglobulin using template-probe double imprinted composites

References

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ref	title	DOI	material type	comment
873	Label-free determination of thyroglobulin using template-probe double imprinted composites	https://doi.org/10.1016/j.snb.2020.128028	Carbon	a novel approach to synthesize molecular imprinting polymers (MIPs)/hemin-graphene nanosheets (H-GNs) composites on the paper, using Tg and 3,3′,5,5′-tetramethylbenzidine (TMB, probe) as double templates

Materials

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ref	material	size	size err	size unit	size type	size comment	BET	b _nanozyme	b 10ⁿ	b unit	specific act	sa 10ⁿ	sa unit	comment
873	H-GNs				XPS	The XPS of the synthesized material further illustrated the construction of MIP composites. Fe2p signals (1.59%) and N1s peak at 398.1 eV of H-GNs/paper were observed, indicating the presence of hemin.

Kinetics

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ref	material	enzyme type	substrate	pH	T	k_m	k_m err	k_m 10ⁿ	k_m unit	v_max	v_max err	v_max 10ⁿ	v_max unit	k_cat	k_cat err	k_cat 10ⁿ	k_cat unit	k_cat/k_m	k_cat/k_m 10ⁿ	k_cat/k_m unit	comment
873	H-GNs	OXD	TMB															873
873	H-GNs	OXD	H2O2			1.25			mM	16.7		-8	M/s					873

Applications

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ref	material	application	target	method	linear range	linear ran unit	LOD	lod unit	recovery	comment
873	H-GNs	It was supposed to be applied for Tg determination in serum to evaluate persistent or recurrent differentiated thyroid carcinoma.	Thyroglobulin (Tg)		0.7-100	ng/mL	0.1	ng/mL