ref | title | DOI | material type | comment |
---|---|---|---|---|
424 | Nanozyme and aptamer-based immunosorbent assay for aflatoxin B1 | https://doi.org/10.1016/j.jhazmat.2020.123154 | Composite | mesoporous SiO2/Au-Pt (m-SAP)/cDNA |
ref | material | size | size err | size unit | size type | size comment | BET | b nanozyme | b 10n | b unit | specific act | sa 10n | sa unit | comment |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
424 | m-SAP/cDNA | 220.82 | nm | TEM | As exhibited in Fig. 3C, the hydrodynamic diameters of m-SiO2 NP, MNP-aptamer and m-SAP/c-DNA are 201.09, 117.95, 220.82 nm, respectively. When MNP-aptamer and m-SAP/cDNA incubated and reacted, the m-SAP/MNP complex generated and showed a large size increase to 457.43 nm, implying the successful hybridization of aptamer and cDNA. |
ref | material | enzyme type | substrate | pH | T | km | km err | km 10n | km unit | vmax | vmax err | vmax 10n | vmax unit | kcat | kcat err | kcat 10n | kcat unit | kcat/km | kcat/km
10n | kcat/km unit | comment |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
424 | m-SAP/cDNA | CAT | H2O2 | 0.016 | mM | 1.473 | -8 | M/s | 424 | ||||||||||||
424 | m-SAP/cDNA | CAT | TMB | 0.058 | mM | 1.279 | -8 | M/s | 424 |
ref | material | application | target | method | linear range | linear ran unit | LOD | lod unit | recovery | comment |
---|---|---|---|---|---|---|---|---|---|---|
424 | m-SAP/cDNA | detection of aflatoxin B1 (AFB1) | Aflatoxin B1 | Color | 0.01-1000 | ng/ml | 5 | pg/ml | 0.042000000000000003 |