Cobalt ferrite nanozyme for efficient symbiotic nitrogen fixation via regulating reactive oxygen metabolism

References

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1223Cobalt ferrite nanozyme for efficient symbiotic nitrogen fixation via regulating reactive oxygen metabolismhttps://doi.org/10.1039/D0EN00935KMetal oxidean antioxidant cobalt ferrite (CoFe2O4) nanozyme as a bridge between nanotechnology and biological nitrogen fixation, which was shown to efficiently regulate the reactive oxygen metabolism and protect nitrogenase

Materials

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1223CoFe2O4

Kinetics

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1223CoFe2O4PODH2O26.550.441μM0.0226μM/min1223

Applications

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1223CoFe2O4an antioxidant cobalt ferrite (CoFe2O4) nanozyme as a bridge between nanotechnology and biological nitrogen fixation, which was shown to efficiently regulate the reactive oxygen metabolism and protect nitrogenase, thereby significantly enhancing the symbiotic nitrogen fixation efficiency by 260% in Glycine max (L.) Merr. (soybean). The CoFe2O4 nanozyme was also revealed to effectively reduce the concentration of ROS in the nodule by 56.6%, creating a superior environment for the proliferation of rhizobia and forming more effective nodules (larger nodules for an increase of 45.6% in the number of parasitic rhizobia). Furthermore, the CoFe2O4 nanozyme was shown to act as a synergist of leghemoglobin and increase its accumulation by 45.9%, where the high concentration of leghemoglobin in nodular cells can create a relatively hypoxic environment and protect nitrogenase, thus achieving a quantitative leap in nitrogen fixation capacity and simultaneously increasing the soybean photosynthesis by 67.2%. Our study has demonstrated that the CoFe2O4 nanozyme can efficiently regulate the intracellular ROS metabolism and serve as a promising strategy for enhancing symbiotic nitrogen fixation.ROS