Electrochemical detection of β-lactoglobulin based on a highly selective DNA aptamer and flower-like Au@ BiVO4 microspheres

References

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title
DOI
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683Electrochemical detection of β-lactoglobulin based on a highly selective DNA aptamer and flower-like Au@ BiVO4 microsphereshttps://doi.org/10.1016/j.aca.2020.04.066CompositeThe flower-like BiVO4 microspheres were firstly found to have peroxidase mimic catalytic activity and used to amplify the electrochemical signal. The aptamer can bind β-lactoglobulin and fall off from the working electrode, after which the DNA2/Au/BiVO4 probe can be fixed to the DNA1/AuNPs/ITO working electrode by the hybridization of DNA2 with DNA1.

Materials

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material
size
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size comment
BET
b nanozyme
b 10n
b unit
specific act
sa 10n
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683BiVO4nmSEMIt is clear that the flower-like BiVO4 microspheres with uniform structure were assembled from BiVO4 nanosheets and were basically consistent with the description in the literature [26].

Kinetics

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pH
T
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vmax
vmax err
vmax 10n
vmax unit
kcat
kcat err
kcat 10n
kcat unit
kcat/km
kcat/km 10n
kcat/km unit
comment
683BiVO4POD683

Applications

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target
method
linear range
linear ran unit
LOD
lod unit
recovery
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683BiVO4detection of β-lactoglobulinβ-lactoglobulinE-chem0.01-1000ng/mL0.007ng/mL