Enhancement of gold nanoclusters-based peroxidase nanozymes for detection of tetracycline

References

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title
DOI
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668Enhancement of gold nanoclusters-based peroxidase nanozymes for detection of tetracyclinehttps://doi.org/10.1016/j.microc.2020.104871MetalIn this study, we report a simple protocol for the synthesis of D-trytophan methyl ester protected AuNCs and exhibit their peroxidase-like activity in a chromogenic reaction coupling with 3,3′,5,5′-tetramethylbenzidine-H2O2.

Materials

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material
size
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BET
b nanozyme
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specific act
sa 10n
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668D-Trp-OMe@AuNCs2.3nmDLSAs shown in Fig. 2a and b, the D-Trp-OMe@AuNCs were monodisperse and spherical with an average size of 2.3 nm. The addition of TC induced the aggregation of D-Trp-OMe@AuNCs to form the D-Trp-OMe@AuNCs-TC composites [28] (Fig. 2d). Fig. 2c clearly shows that 24.2 nm was the average size of the D-Trp-OMe@AuNCs-TC composites.

Kinetics

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substrate
pH
T
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vmax
vmax err
vmax 10n
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kcat
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kcat/km unit
comment
668D-Trp-OMe@AuNCs-TCPODH2O20.011.748-8M/s668
668D-Trp-OMe@AuNCs-TCPODTMB0.2541.923-8M/s668
668D-Trp-OMe@AuNCsPODH2O20.0570.831-8M/s668
668D-Trp-OMe@AuNCsPODTMB0.1820.192-8M/s668

Applications

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target
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linear range
linear ran unit
LOD
lod unit
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668D-Trp-OMe@AuNCsdetection of trtracyclineTMBColor1.5-30.0μM0.20μM99.0-105.0%