ref | title | DOI | material type | comment |
---|---|---|---|---|
779 | A Phosphatase‐Mimetic Nano‐Stabilizer of Mast Cells for Long‐Term Prevention of Allergic Disease | https://doi.org/10.1002/advs.202004115 | Metal oxide | ceria nanoparticle (CeNP-) based phosphatase-mimetic nano-stabilizers (PMNSs) |
ref | material | enzyme type | substrate | pH | T | km | km err | km 10n | km unit | vmax | vmax err | vmax 10n | vmax unit | kcat | kcat err | kcat 10n | kcat unit | kcat/km | kcat/km
10n | kcat/km unit | comment |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
779 | PMNSs | hydrolase | O-phospho-L-tyrosine (P-Tyr) | 4.23 | -6 | M | 1.92 | -9 | M/s | 779 | The phosphatase-mimetic activity of PMNSs at different Ce concentrations (5,10, 20, and 40 μg/mL) or different P-Tyr concentrations (9, 18, 36, and 72 μM) was detected by the malachite green assay (Sangon Biotech Co., Ltd., China). Theabsorbance of the malachite green solution containing P-Tyr was used as blank. A phosphate standard curve was obtainedby using KH2PO4as the substrate |