Nanozyme-linked immunosorbent assay for porcine circovirus type 2 antibody using HAuCl4/H2O2 coloring system

References

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664Nanozyme-linked immunosorbent assay for porcine circovirus type 2 antibody using HAuCl4/H2O2 coloring systemhttps://doi.org/10.1016/j.microc.2020.105079CompositeHerein, a new HAuCl4/H2O2 coloring system was proposed for naked-eye detection of porcine circovirus type 2 (PCV2) antibody using nanozyme, Au-Pt/SiO2 as labels. Be similar to the oxidation capacity of H2O2 towards TMB, HAuCl4 can also be reduced into gold nanoparticles (Au NPs) by H2O2.

Materials

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BET
b nanozyme
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664Au-Pt/SiOTEMAs shown in Fig. S1A, SiO2 NPs are homogenous with good monodispersity. After conjugating with Au NPs (∼5 nm), small black dots are evenly covered on the surface of SiO2 NPs, resulting with the Au/SiO2 hybrids (Fig. S1B).

Kinetics

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664Au-Pt/SiOPOD664

Applications

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linear range
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664NLISA-Hdetection of serumHAuCl4Color0.1-10ng/mL0.05
664NLISA-Tdetection of serumTMBColor0.1-10ng/mL0.08ng/mL