One-step fabrication of trimetallic alloy nanozyme catalyzer for luminol-H2O2 chemiluminescence and its application for miRNA-21 detection coupled with miRNA walking machine

References

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448One-step fabrication of trimetallic alloy nanozyme catalyzer for luminol-H2O2 chemiluminescence and its application for miRNA-21 detection coupled with miRNA walking machinehttps://doi.org/10.1016/j.jpba.2020.113280PtCuCo trimetallic alloys (PtCuCo-TAs)

Materials

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448PtCuCo-TAsnmSEMNote that the obtained pure Pt nanoparticles had 3D network-like porous nanostructures, as shown in Fig. 1A. And the diameters of Pt nanoparticles were ranging from 8 to 12 nm. Compared with the 3D network-like porous nanostructures of Pt, the pure Cu nanomaterials were rough and composed of many 3D irregular spherical nanoparticles with porous structures and the diameters of Cu nanomaterials were about 45 ± 3 nm as shown in Fig. 1B. Furthermore, Fig. 1C exhibited the morphology of Co nanomaterials, and it was found that Co nanomaterials displayed uniform flower-like nanostructures with diameter up to 160 ± 10 nm. Simultaneously, the images of Fig. 1D revealed that PtCuCo-TAs had irregular bulk structure. The results of morphology for different samples revealed that PtCuCo-TAs was synthesized successfully.

Kinetics

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Applications

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448PtCuCo-TAsmiRNA-21 detectionmiRNA-21CL5 × 10−16 - 1 × 10-10M1.67 × 10−16M99.0–104.4%
448PtCuCo-TAsmiRNA-21 detectionmiRNA-21CL5 × 10−16 - 1 × 10-10M1.67 × 10−16M99.0–104.4%this method can effectively apply for the detection of miRNA-21 with accuracy and reliability in sample analysis.