| 5073 |
166 |
CB-CQDs |
The detection of biothiols was performed as follows: in a series of colorimetric tubes, 0.5 mL of TMB (20 mM), 0.5 mL of H2O2 (25 mM), and 0.1 mL of CB-CQDs were fully mixed in 3.8 mL of HAc-NaAc buffer at pH4.5. Then, various concentrations of biothiols standard solution (0.1 mL) were added into the above mixture. After they were well mixed and incubated at 40 °C for 25 min, the absorption spectra were recorded on a Unico 4802 ultraviolet-visible spectrophotometer at room temperature. The calibration curves for biothiols were established according to the decrease of absorbance defined as ΔA=A0﹣A, where A0 and A denote the absorbance at 652 nm without and with analyte, individually. |
cysteine |
Color |
0.5-20 |
μM |
0.4 |
μM |
95.9±2.7 |
105.7±2.0； 109.3±1.1； 99.7±4.3； 91.5±1.0； 98.2±2.3 |
| 5074 |
166 |
CB-CQDs |
The detection of biothiols was performed as follows: in a series of colorimetric tubes, 0.5 mL of TMB (20 mM), 0.5 mL of H2O2 (25 mM), and 0.1 mL of CB-CQDs were fully mixed in 3.8 mL of HAc-NaAc buffer at pH4.5. Then, various concentrations of biothiols standard solution (0.1 mL) were added into the above mixture. After they were well mixed and incubated at 40 °C for 25 min, the absorption spectra were recorded on a Unico 4802 ultraviolet-visible spectrophotometer at room temperature. The calibration curves for biothiols were established according to the decrease of absorbance defined as ΔA=A0﹣A, where A0 and A denote the absorbance at 652 nm without and with analyte, individually. |
cysteine |
Color |
0.5-20 |
μM |
0.4 |
μM |
95.9±2.7 |
|