Nanozyme Database

	ref	material	size	size err	size unit	size type	size comment	BET	b _nanozyme	b 10ⁿ	b unit	specific act	sa 10ⁿ	sa unit	comment

	ref	material	enzyme type	substrate	pH	T	k_m	k_m err	k_m 10ⁿ	k_m unit	v_max	v_max err	v_max 10ⁿ	v_max unit	k_cat	k_cat err	k_cat 10ⁿ	k_cat unit	k_cat/k_m	k_cat/k_m 10ⁿ	k_cat/k_m unit	comment

	ref	material	application	target	method	linear range	linear ran unit	LOD	lod unit	recovery	comment
5300	390	Nanozymes with hard coronas (Corona-NZ)	We observed that the structure of the AuNP ligands dictates the formation of protein coronas and selectively controls catalytic activity of nanozymes. A hard “irreversible” corona (without TEG) deactivated nanozymes through aggregation and steric blocking, while a soft “reversible” corona (with TEG) partially reduced the catalytic activity. The catalytic activity of both soft and hard nanozymes was restored after proteolytic degradation of the protein corona through endogenous proteases present in the endosome and lysosome. Hence, a selective intracellular activation system (without TEG) and an always-on system (with TEG) are obtained by engineering the monolayer of ligands on nanoparticles. This study provides a direct and versatile approach for specific activation of bioorthogonal catalysts through tuning the formation of the protein corona on nanozymes. This approach has the potential to reduce the off-target effect and extend on-demand generation of imaging agents and localized therapeutics. The generality of this system is suitable for in vivo applications, which are currently under investigations in our group.

	ref	title	DOI	material type	comment