VONP-LPs

Materials

ref material size size err size unit size type size comment BET b nanozyme b 10n b unit specific act sa 10n sa unit comment
7457 165 VONP-LPs 25 1.5 nm TEM the particle size distribution of V2O5 NPs in the range of 15–40 nm with average lateral size of 25 � 1.5 nm.

Kinetics

ref material enzyme type substrate pH T km km err km 10n km unit vmax vmax err vmax 10n vmax unit kcat kcat err kcat 10n kcat unit kcat/km kcat/km 10n kcat/km unit comment
6053 165 VONP-LPs POD H2O2 0.04 mM 20.9 -1 μM/min 165 M-1 s-1
6052 165 VONP-LPs POD TMB 0.02 mM 19.7 -1 μM/min 165 M-1 s-1 165

Applications

ref material application target method linear range linear ran unit LOD lod unit recovery comment
5072 165 VONP-LPs To determine the linear range and sensitivity of the developed dualmodality sensor, different concentrations of NoV-LPs are examined. Anti-NoV antibody-conjugated VONP-LPs, MNPs and aliquot of NoV-LPs with various concentrations are mixed. VONP-LPs and the MNPs are bound with NoV-LPs through the specific interaction with antibody on their surface and a nanoconjugate of VONP-LPs, NoV-LPs and MNPs is formed. NoV-LPs Color 10-108 fg/ml 4.1 fg/ml
5071 165 VONP-LPs Above results confirmed the ultra sensitivity and excellent specificity of the VONP-LPs based dual-modality biosensor proving applicability of developed sensor for real samples (Fig. 5b). To confirm the practicability real clinical samples are examined. Different types of clinical NoV (GII. 2, GII. 3, GII.4) from human feces of infected patients are detected using the developed dual-modality sensor NoV-LPs and clinical samples Color 100-107 copies/ml 72 copies/ml

References

ref title DOI material type comment
3396 165 Dual modality sensor using liposome-based signal amplification technique for ultrasensitive norovirus detection https://doi.org/10.1016/j.bios.2020.112169 Metal oxide V2O5 nanoparticles-encapsulated liposomes (VONP-LPs)