aptamers@BSA-AuNCs

Materials

ref material size size err size unit size type size comment BET b nanozyme b 10n b unit specific act sa 10n sa unit comment
7745 515 aptamers@BSA-AuNCs 1.77 0.51 nm TEM The average diameters of BSA-AuNCs and aptamers@BSA-AuNCs are 1.79 ± 0.52 nm and 1.77 ± 0.51 nm, respectively (Figs. S2B and S2D).

Kinetics

ref material enzyme type substrate pH T km km err km 10n km unit vmax vmax err vmax 10n vmax unit kcat kcat err kcat 10n kcat unit kcat/km kcat/km 10n kcat/km unit comment
6381 515 aptamers@BSA-AuNCs POD H2O2 5.5 37 40.7 mM 14.47 -8 M/s 515 As shown in Table 1, with H2O2 as the substrate, the Km value of bacteria@aptamer1,2@BSA-AuNCs was similar to that of aptamer1,2@BSAAuNCs, demonstrating that the enhancement of the peroxidase-like activity of aptamer1,2@BSA-AuNCs did not originate from the increased affinity for H2O2. In comparison with aptamer1,2@BSA-AuNCs, the Vmax value of bacteria@aptamer1,2@BSA-AuNCs exhibited about 3 times improvement, which might be attributed to the enrichment of bacteria toward aptamer1,2@BSA-AuNCs and TMB, thus promoting the proximity of aptamer1,2@BSA-AuNCs to TMB and greatly increasing the local concentrations of the enzyme-mimetic nanoparticles and their substrate.
6380 515 aptamers@BSA-AuNCs POD TMB 5.5 37 0.3 mM 17.42 -8 M/s 515 515

Applications

ref material application target method linear range linear ran unit LOD lod unit recovery comment
5458 515 aptamers@BSA-AuNCs for colorimetric detection of Salmonella typhimurium Salmonella typhimurium Color 101-106 cfu/mL 1 cfu/mL 92.4% - 110%

References

ref title DOI material type comment
3702 515 Enhancement of the peroxidase-like activity of aptamers modified gold nanoclusters by bacteria for colorimetric detection of Salmonella typhimurium https://doi.org/10.1016/j.talanta.2020.121476 Metal dual aptamers modified bovine serum albumin stabilized-gold nanoclusters (aptamers@BSA-AuNCs)