Label-free homogeneous electrochemical detection of MicroRNA based on target-induced anti-shielding against the catalytic activity of two-dimension nanozyme

References

ref title DOI material type comment
3515 317 Label-free homogeneous electrochemical detection of MicroRNA based on target-induced anti-shielding against the catalytic activity of two-dimension nanozyme https://doi.org/10.1016/j.bios.2020.112707 Metal oxide Herein, a soft template-directed wet chemical approach was proposed for preparation of 2D MnO2 nanoflakes, in which the morphology can be easily tuned by the template dosage.

Materials

ref material size size err size unit size type size comment BET b nanozyme b 10n b unit specific act sa 10n sa unit comment
7559 317 2D MnO2 nanoflakes 300*5 nm TEM The lateral dimension and thickness of 2D MnO2 nanoflakes were calculated to be 300 nm and 5 nm, respec

Kinetics

ref material enzyme type substrate pH T km km err km 10n km unit vmax vmax err vmax 10n vmax unit kcat kcat err kcat 10n kcat unit kcat/km kcat/km 10n kcat/km unit comment
6199 317 2D MnO2 nanoflakes POD TMB/OPD/ABTS, 317

Applications

ref material application target method linear range linear ran unit LOD lod unit recovery comment
5208 317 2D MnO2 nanoflakes detect microRNA Let-7a E-chem 0.4 to 100 nM 250 pM 105.4%, 96.3%, and 102.1%