A colorimetric immunoassay for determination of Escherichia coli O157: H7 based on oxidase-like activity of cobalt-based zeolitic imidazolate framework

References

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title
DOI
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1347A colorimetric immunoassay for determination of Escherichia coli O157: H7 based on oxidase-like activity of cobalt-based zeolitic imidazolate frameworkhttps://doi.org/10.1007/s00604-020-04407-3CompositePreparation of the immunoprobe involved self-polymerized dopamine being applied for the surface modification of ZIF-67 nanosheets in order to bind to the antibody(ZIF-67@PDA@antibody)

Materials

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size
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BET
b nanozyme
b 10n
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specific act
sa 10n
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1347Fe3O4@PDA40nmTEM
1347Fe3O4@PDA100nmSEM
1347ZIF-67 nanosheets400nmSEMThe morphology of ZIF-67 nanosheets shows irregular flakes that are 400 nm in diameter. Modification of dopamine on the surface of ZIF-67 nanosheets did not change its morphology.

Kinetics

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T
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kcat
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kcat 10n
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kcat/km 10n
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Applications

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linear range
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LOD
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1347ZIF-67@PDAdetermination of Escherichia coli O157:H7Escherichia coli O157:H7Color30-3.0×10^8CFU/mL12CFU/mL