Nanozyme Database

	ref	title	DOI	material type	comment
3409	178	Biogenic synthesis of AuPd nanocluster as a peroxidase mimic and its application for colorimetric assay of acid phosphatase	https://doi.org/10.1016/j.colsurfa.2020.124444	Multi-metal	AuPt nanoclusters (Au1Pd5 NCs)

	ref	material	size	size err	size unit	size type	size comment	BET	b _nanozyme	b 10ⁿ	b unit	specific act	sa 10ⁿ	sa unit	comment
7468	178	Au 1 Pd 5	1.4		nm	TEM	TEM was performed to investigate the size distribution of monometallic and bimetallic NCs. As shown in Fig. 2a and b, NADH-reduced Pd NCs with 24 h-incubation exhibit the mean size of 1.1 nm, while Au NPs show the average diameter of 8.1 nm. Remarkably, both Au1Pd5 and Au1Pd1 exist as highly dispersed NCs, showing the average size of 1.4 and 1.6 nm respectively (Fig. 2c and d). The mean size increases in the order of Pd < Au1Pd5 < Au1Pd1 < Au, which is consistent with the relative content of Au species. Hence, it is reasonable that NADH facilitates the rapid formation of ultrasmall NCs within a suitable range of [Na2PdCl4]/[HAuCl4]. Further, the atomic ratio in bimetallic NCs was determined by ICP-OES. The molar ratio of [Au]/[Pd] is 1.3 for Au1Pd1 and 0.25 for Au1Pd5, which is close to the corresponding theoretical ratio of two precursors in synthesis process.

	ref	material	enzyme type	substrate	pH	T	k_m	k_m err	k_m 10ⁿ	k_m unit	v_max	v_max err	v_max 10ⁿ	v_max unit	k_cat	k_cat err	k_cat 10ⁿ	k_cat unit	k_cat/k_m	k_cat/k_m 10ⁿ	k_cat/k_m unit	comment
6070	178	Au1Pd5 NCs	POD	H2O2			160			mM	8.72		-8	M/s					178
6069	178	Au1Pd5 NCs	POD	TMB			0.086			mM	6.48		-8	M/s					178

	ref	material	application	target	method	linear range	linear ran unit	LOD	lod unit	recovery	comment
5086	178	Au 1 Pd 5	A colorimetric test is developed for quantitative determination of acid phosphatase.	Acid phosphatase (ACP)	Color	1-14	U/L	0.53	U/L	102% 103% 99%	To validate the application of this method in human serum, spiked-recovery experiments were carried out with different concentration of ACP. To fit the linear range of the established calibration plot, commercial human serum was appropriately diluted before addition of ACP. The recovery rates are 102 % for 4 U/L, 103 % for 8 U/L and 99 % for 12 U/L ACP (listed in Table 1). The good recovery results guarantee the reliability of this method for estimating ACP activity in biological fluid.
5085	178	Au 1 Pd 5	A colorimetric test is developed for quantitative determination of acid phosphatase.	Acid phosphatase (ACP)	Color	1-14	U/L	0.53	U/L	102% 103% 99%