Nanozyme-linked immunosorbent assay for porcine circovirus type 2 antibody using HAuCl4/H2O2 coloring system

References

ref title DOI material type comment
3850 664 Nanozyme-linked immunosorbent assay for porcine circovirus type 2 antibody using HAuCl4/H2O2 coloring system https://doi.org/10.1016/j.microc.2020.105079 Composite Herein, a new HAuCl4/H2O2 coloring system was proposed for naked-eye detection of porcine circovirus type 2 (PCV2) antibody using nanozyme, Au-Pt/SiO2 as labels. Be similar to the oxidation capacity of H2O2 towards TMB, HAuCl4 can also be reduced into gold nanoparticles (Au NPs) by H2O2.

Materials

ref material size size err size unit size type size comment BET b nanozyme b 10n b unit specific act sa 10n sa unit comment
7881 664 Au-Pt/SiO TEM As shown in Fig. S1A, SiO2 NPs are homogenous with good monodispersity. After conjugating with Au NPs (∼5 nm), small black dots are evenly covered on the surface of SiO2 NPs, resulting with the Au/SiO2 hybrids (Fig. S1B).

Kinetics

ref material enzyme type substrate pH T km km err km 10n km unit vmax vmax err vmax 10n vmax unit kcat kcat err kcat 10n kcat unit kcat/km kcat/km 10n kcat/km unit comment
6547 664 Au-Pt/SiO POD 664

Applications

ref material application target method linear range linear ran unit LOD lod unit recovery comment
5594 664 NLISA-H detection of serum HAuCl4 Color 0.1-10 ng/mL 0.05
5593 664 NLISA-T detection of serum TMB Color 0.1-10 ng/mL 0.08 ng/mL