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Dramatically Enhanced Immunochromatographic Assay Using Cascade Signal Amplification for Ultrasensitive Detection of Escherichia coli O157:H7 in Milk

References

ref title DOI material type comment
3309 78 Dramatically Enhanced Immunochromatographic Assay Using Cascade Signal Amplification for Ultrasensitive Detection of Escherichia coli O157:H7 in Milk https://doi.org/10.1021/acs.jafc.9b07076 Metal AuNP-ICA platform

Materials

ref material size size err size unit size type size comment BET b nanozyme b 10n b unit specific act sa 10n sa unit comment
7384 78 AuNP 38 nm TEM average diameter

Kinetics

ref material enzyme type substrate pH T km km err km 10n km unit vmax vmax err vmax 10n vmax unit kcat kcat err kcat 10n kcat unit kcat/km kcat/km 10n kcat/km unit comment
5976 78 AuNP-AA POD TMB 4 RT 0.27 mM 32.52 -9 M/s 1145.17 0 1/s 78 -3 M-1 s-1 78
5975 78 AuNP-HQ POD H2O2 4 RT 0.16 mM 20.16 -9 M/s 724.64 0 1/s 78 -3 M-1 s-1 78
5974 78 AuNP-NH2OH POD TMB 4 RT 0.58 mM 63.54 -9 M/s 2218.30 0 1/s 78 -3 M-1 s-1 78
5973 78 AuNP-AA POD H2O2 4 RT 0.2 mM 26.24 -9 M/s 924.09 0 1/s 78 -3 M-1 s-1 78
5972 78 AuNP-HQ POD TMB 4 RT 0.23 mM 25.95 -9 M/s 913.59 0 1/s 78 -3 M-1 s-1 78
5971 78 AuNP-NH2OH POD H2O2 4 RT 0.41 mM 36.05 -9 M/s 1269.33 0 1/s 78 -3 M-1 s-1 78

Applications

ref material application target method linear range linear ran unit LOD lod unit recovery comment
4994 78 AuNP-ICA platform Detection of Escherichia coli O157:H7 in Milk E. coli Color 5-2.5*10^5 CFU/mL 12.5 CFU/mL 90.94


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