Heparin-platinum nanozymes with enhanced oxidase-like activity for the colorimetric sensing of isoniazid

References

ref title DOI material type comment
3402 171 Heparin-platinum nanozymes with enhanced oxidase-like activity for the colorimetric sensing of isoniazid https://doi.org/10.1016/j.talanta.2019.120707 Metal heparin sodium stabilized platinum nanoparticles (HS-PtNPs)

Materials

ref material size size err size unit size type size comment BET b nanozyme b 10n b unit specific act sa 10n sa unit comment
7462 171 HS-PtNPs 4.8 0.6 nm TEM TEM image shows that the average diameter of HS-PtNPs was 4.8 ± 0.6 nm (Fig. 1C), and the high resolution TEM (HRTEM) image shows that HS-PtNPs possessed a 0.30 nm continuous lattice spacing 2819.16 U/g

Kinetics

ref material enzyme type substrate pH T km km err km 10n km unit vmax vmax err vmax 10n vmax unit kcat kcat err kcat 10n kcat unit kcat/km kcat/km 10n kcat/km unit comment
6060 171 HS-PtNPs OXD TMB 0.01012 mM 171 171

Applications

ref material application target method linear range linear ran unit LOD lod unit recovery comment
5080 171 HS-PtNPs These obvious advantages prompted us to explore the practical use of HS-PtNPs. The pyridine ring of isoniazid has strong reductive hydrazyl substitution, which can compete with TMB for the catalytic site of HS-PtNPs (Scheme 1). The introduction of isoniazid in HS-PtNPs-catalyzed oxidation process of TMB results in a lower efficiency and colorless reaction in TMB oxidation. isoniazid Color 2.5-250 μM 1.7 μM 95%-103%