A thiamine-triggered fluormetric assay for acetylcholinesterase activity and inhibitor screening based on oxidase-like activity of MnO2 nanosheets

References

ref title DOI material type comment
3809 623 A thiamine-triggered fluormetric assay for acetylcholinesterase activity and inhibitor screening based on oxidase-like activity of MnO2 nanosheets https://doi.org/10.1016/j.talanta.2020.121362 Composite manganese dioxide nanosheets (MnO2 NSs)

Materials

ref material size size err size unit size type size comment BET b nanozyme b 10n b unit specific act sa 10n sa unit comment
7845 623 MnO2 NSs nm TEM As presented in Fig. 1A, the synthetic MnO2 NSs exhibited an ultrathin 2D sheetlike structure.

Kinetics

ref material enzyme type substrate pH T km km err km 10n km unit vmax vmax err vmax 10n vmax unit kcat kcat err kcat 10n kcat unit kcat/km kcat/km 10n kcat/km unit comment
6508 623 thiamine-MnO2 OXD ATCh 623 623

Applications

ref material application target method linear range linear ran unit LOD lod unit recovery comment
5560 623 thiamine-MnO2 A thiamine-triggered fluormetric assay for acetylcholinesterase activity and inhibitor screening acetylcholinesterase (AChE) Fluor 0.02-1 mU/mL 15 μU/mL