ref | title | DOI | material type | comment |
---|---|---|---|---|
181 | Hemin@carbon dot hybrid nanozymes with peroxidase mimicking properties for dual (colorimetric and fluorometric) sensing of hydrogen peroxide, glucose and xanthine | https://doi.org/10.1007/s00604-019-4103-4 | Composite | hemin@carbon dot hybrid nanozymes (hemin@CD) |
ref | material | size | size err | size unit | size type | size comment | BET | b nanozyme | b 10n | b unit | specific act | sa 10n | sa unit | comment |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
181 | hemin@CD | 2.3 | nm | TEM | High-resolution TEM image shows the lattice fringe of 0.21 nm corresponding to the (100) facet (inset of Fig. 1a) [27]. After hemin was modified on CDs, the average size of hemin@CDs is 2.3 nm (Fig. 1b), which suggests there have been no significant change in the average size of CDs after hemin molecule decoration. |
ref | material | enzyme type | substrate | pH | T | km | km err | km 10n | km unit | vmax | vmax err | vmax 10n | vmax unit | kcat | kcat err | kcat 10n | kcat unit | kcat/km | kcat/km
10n | kcat/km unit | comment |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
181 | hemin@CD | POD | H2O2 | 0.3 | mM | 181 | 181 | ||||||||||||||
181 | hemin@CD | POD | 4-APP | 0.011 | mM | 181 | 181 | ||||||||||||||
181 | hemin@CD | POD | phenol | 0.031 | mM | 181 | Fig. S4 shows that the apparent Km values with phenol, 4-APP and H2O2 are 0.031 mM, 0.011 mM, and 0.30 mM, respectively. The apparent Km value of hemin@CDs with phenol is lower than that of HRP (5 mM) [32], suggesting higher affinity for phenol. |
ref | material | application | target | method | linear range | linear ran unit | LOD | lod unit | recovery | comment |
---|---|---|---|---|---|---|---|---|---|---|
181 | hemin@CD | a colorimetric and fluorescent dual-channel sensor for H2O2, glucose and xanthine was developed, and the results are satisfied in the application of real samples | H2O2 | Color | 0.17–133 | μM | 0.11 | μM | ||
181 | hemin@CD | a colorimetric and fluorescent dual-channel sensor for H2O2, glucose and xanthine was developed, and the results are satisfied in the application of real samples | glucose | Color | 0.17–133 | μM | 0.15 | μM | 92.2%~105.6% | |
181 | hemin@CD | a colorimetric and fluorescent dual-channel sensor for H2O2, glucose and xanthine was developed, and the results are satisfied in the application of real samples | H2O2 | Fluor | 0.17–133 | μM | 0.15 | μM | ||
181 | hemin@CD | a colorimetric and fluorescent dual-channel sensor for H2O2, glucose and xanthine was developed, and the results are satisfied in the application of real samples | xanthine | Color | 0.17–33 | μM | 0.15 | μM | 93.4-102.4% | |
181 | hemin@CD | a colorimetric and fluorescent dual-channel sensor for H2O2, glucose and xanthine was developed, and the results are satisfied in the application of real samples | xanthine | Fluor | 0.17–33 | μM | 0.12 | μM | 98.8-103.6% | |
181 | hemin@CD | a colorimetric and fluorescent dual-channel sensor for H2O2, glucose and xanthine was developed, and the results are satisfied in the application of real samples | glucose | Fluor | 0.17–133 | μM | 0.15 | μM | 92.2%~105.6% |