Hemin@carbon dot hybrid nanozymes with peroxidase mimicking properties for dual (colorimetric and fluorometric) sensing of hydrogen peroxide, glucose and xanthine

References

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181Hemin@carbon dot hybrid nanozymes with peroxidase mimicking properties for dual (colorimetric and fluorometric) sensing of hydrogen peroxide, glucose and xanthinehttps://doi.org/10.1007/s00604-019-4103-4Compositehemin@carbon dot hybrid nanozymes (hemin@CD)

Materials

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181hemin@CD2.3nmTEMHigh-resolution TEM image shows the lattice fringe of 0.21 nm corresponding to the (100) facet (inset of Fig. 1a) [27]. After hemin was modified on CDs, the average size of hemin@CDs is 2.3 nm (Fig. 1b), which suggests there have been no significant change in the average size of CDs after hemin molecule decoration.

Kinetics

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181hemin@CDPODH2O20.3mM181181
181hemin@CDPOD4-APP0.011mM181181
181hemin@CDPODphenol0.031mM181Fig. S4 shows that the apparent Km values with phenol, 4-APP and H2O2 are 0.031 mM, 0.011 mM, and 0.30 mM, respectively. The apparent Km value of hemin@CDs with phenol is lower than that of HRP (5 mM) [32], suggesting higher affinity for phenol.

Applications

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181hemin@CDa colorimetric and fluorescent dual-channel sensor for H2O2, glucose and xanthine was developed, and the results are satisfied in the application of real samplesH2O2Color0.17–133μM0.11μM
181hemin@CDa colorimetric and fluorescent dual-channel sensor for H2O2, glucose and xanthine was developed, and the results are satisfied in the application of real samplesglucoseColor0.17–133μM0.15μM92.2%~105.6%
181hemin@CDa colorimetric and fluorescent dual-channel sensor for H2O2, glucose and xanthine was developed, and the results are satisfied in the application of real samplesH2O2Fluor0.17–133μM0.15μM
181hemin@CDa colorimetric and fluorescent dual-channel sensor for H2O2, glucose and xanthine was developed, and the results are satisfied in the application of real samplesxanthineColor0.17–33μM0.15μM93.4-102.4%
181hemin@CDa colorimetric and fluorescent dual-channel sensor for H2O2, glucose and xanthine was developed, and the results are satisfied in the application of real samplesxanthineFluor0.17–33μM0.12μM98.8-103.6%
181hemin@CDa colorimetric and fluorescent dual-channel sensor for H2O2, glucose and xanthine was developed, and the results are satisfied in the application of real samplesglucoseFluor0.17–133μM0.15μM92.2%~105.6%