Emission Wavelength Switchable Carbon Dots Combined with Biomimetic Inorganic Nanozymes for a Two-Photon Fluorescence Immunoassay

References

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875Emission Wavelength Switchable Carbon Dots Combined with Biomimetic Inorganic Nanozymes for a Two-Photon Fluorescence Immunoassayhttps://doi.org/10.1021/acsami.0c06240Compositeo-phenylenediamine (oPD) was designed as the precursor to prepare yellow fluorescent carbon dots (named o-CDs) by the solvothermal method. The fluorescence of o-CDs can be quenched by ferrous ion (Fe2+); after adding H2O2 into the o-CDs+Fe2+ system, the fluorescence is recovered and the fluorescence color of o-CDs is changed from yellow to green.

Materials

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875DMSN-Au NP17.7nmTEM407.8875

Kinetics

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875DMSN-Au NPOXD6.80.13mM3.97μM/min22.431/s875

Applications

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875DMSN-Au NPglucose oxidase by DMSN-Au NPs not only reduces the cost but also provides significantly amplified signals due to DMSNs haing a high specific surface area. We show the detection of carcinoembryonic antigen (CEA) as an example target to evaluate the analytical figure of merits of the proposed strategy. Under the optimal conditions, twophoton- based o-CDs displayed excellent performances for CEA0.1-80ng/mL74.5pg/mL