Histidine capped-gold nanoclusters mediated fluorescence detection of glucose and hydrogen peroxide based on glucose oxidase-mimicking property of gold nanoparticles via an …

References

ref title DOI material type comment
3993 807 Histidine capped-gold nanoclusters mediated fluorescence detection of glucose and hydrogen peroxide based on glucose oxidase-mimicking property of gold nanoparticles via an … https://doi.org/10.1016/j.jlumin.2020.117604 Metal gold nanoparticles (AuNPs)

Materials

ref material size size err size unit size type size comment BET b nanozyme b 10n b unit specific act sa 10n sa unit comment
8014 807 AuNPs 25 nm TEM The morphology of the His-AuNCs was studied via their TEM images taken. As sit is seen from Fig. 1A, the average diameter of the synthesized His-AuNCs is about 2 nm and their morphology and size are nearly spherical and uniform. The TEM images were also utilized to estimate the average diameters of the enlarged AuNPs seeds in the presence of glucose (Fig. 1B). The average diameters of His-AuNPs seeds were 10 ± 2 nm, while the diameter of enlarged AuNPs depend on the concentration of glucose and self-catalyzed activity of AuNPs. The TEM images reveal that the AuNPs in the presence of 50 μM glucose can be enlarged to an average size of 17 nm (Fig. 1C), while the diameter of enlarged AuNPs in the presence of higher glucose concentrations of 100 μM further increased to about 25 nm (Fig. 1D).

Kinetics

ref material enzyme type substrate pH T km km err km 10n km unit vmax vmax err vmax 10n vmax unit kcat kcat err kcat 10n kcat unit kcat/km kcat/km 10n kcat/km unit comment
6712 807 AuNPs OXD Glucose 24.6 mM 57.4 -8 M/s 807

Applications

ref material application target method linear range linear ran unit LOD lod unit recovery comment
5733 807 AuNPs detection of hydrogen peroxide H2O2 Color 5-135 μM 3.6 μM
5732 807 AuNPs determination of glucose glucose Color 5-125 μM 3.4 μM 99.0-101.6% The IFE-based assay system was utilized for determining the levels of glucose in urine samples of a diabetic patients.