A Visual and Sensitive Detection of Escherichia coli Based on Aptamer and Peroxidase-like Mimics of Copper-Metal Organic Framework Nanoparticles

References

ref title DOI material type comment
4057 871 A Visual and Sensitive Detection of Escherichia coli Based on Aptamer and Peroxidase-like Mimics of Copper-Metal Organic Framework Nanoparticles https://doi.org/10.1007/s12161-020-01765-9 MOF Cu-MOF NPs were synthesized and functionalized with streptavidin and biotinylated aptamer 2 to form the signal probes

Materials

ref material size size err size unit size type size comment BET b nanozyme b 10n b unit specific act sa 10n sa unit comment
8066 871 Cu-MOF TEM, SEM The morphology of prepared Cu-MOF NPs was characterized by TEMand SEManalysis. As displayed in Fig. 2a, b, the Cu- MOF NPs have a spherical shape with uniform particle distribution.

Kinetics

ref material enzyme type substrate pH T km km err km 10n km unit vmax vmax err vmax 10n vmax unit kcat kcat err kcat 10n kcat unit kcat/km kcat/km 10n kcat/km unit comment
6769 871 Cu-MOF POD H2O2 871
6764 871 Cu-MOF POD H2O2 0.099 mM 21.5 -8 M/s 871

Applications

ref material application target method linear range linear ran unit LOD lod unit recovery comment
5798 871 Cu-MOF This peroxidase-like mimics-based colorimetric aptasensor showed a rapid and sensitive quantification of E. coli E. coli 16~1.6 × 106 cfu/mL 2 cfu/mL