Self-assembled chromogen-loaded polymeric cocoon for respiratory virus detection

References

ref title DOI material type comment
4350 1165 Self-assembled chromogen-loaded polymeric cocoon for respiratory virus detection https://doi.org/10.1039/D0NR06893D Metal copper nanoflowers (CuNFs)

Materials

ref material size size err size unit size type size comment BET b nanozyme b 10n b unit specific act sa 10n sa unit comment
8210 1165 CuNFs 400-500 nm SEM The SEM image in Fig. 1h shows the flower-like structures of CuNFs with an average size of 400–500 nm, having hierarchical structures with high surface-to-volume ratios.

Kinetics

ref material enzyme type substrate pH T km km err km 10n km unit vmax vmax err vmax 10n vmax unit kcat kcat err kcat 10n kcat unit kcat/km kcat/km 10n kcat/km unit comment
7127 1165 CuNFs POD TMB 0.07 mM 0.12 -3 mM/s 1165
7126 1165 CuNFs POD H2O2 6.5 mM 0.02 -3 mM/s 1165

Applications

ref material application target method linear range linear ran unit LOD lod unit recovery comment
6059 1165 CuNFs immunoassay demonstrates high sensitivity for IV/A influenza virus (IV)/A 10–10*6 fg mL−1 32.37 fg mL−1 a limit of detection (LOD) as low as 32.37 fg mL−1 and 54.97 fg mL−1 in buffer and serum, respectively. For practical needs, a clinically isolated IV/A/H3N2 and spike protein of SARS-CoV-2 were detected with the LODs of 17 pfu mL−1 and 143 fg mL−1, respectively.