White Peroxidase‐Mimicking Nanozymes: Colorimetric Pesticide Assay without Interferences of O2 and Color

References

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ref
title
DOI
material type
comment
200White Peroxidase‐Mimicking Nanozymes: Colorimetric Pesticide Assay without Interferences of O2 and Colorhttps://doi.org/10.1002/adfm.202001933Metal oxideGeO2

Materials

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material
size
size err
size unit
size type
size comment
BET
b nanozyme
b 10n
b unit
specific act
sa 10n
sa unit
comment
200GeO2showed besom-like morphology with uniform size (width of ≈100 nm and length of ≈1 µm). the “head of besom” was composed of long strip with width of ≈10 nm (Figure 1c,d).

Kinetics

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material
enzyme type
substrate
pH
T
km
km err
km 10n
km unit
vmax
vmax err
vmax 10n
vmax unit
kcat
kcat err
kcat 10n
kcat unit
kcat/km
kcat/km 10n
kcat/km unit
comment
200GeO2PODH2O21.75mM23.400-8M/s200200
200GeO2PODTMB0.42mM23.397-8M/s200

Applications

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material
application
target
method
linear range
linear ran unit
LOD
lod unit
recovery
comment
200GeO2Colorimetric Assay of OPsparaoxonColor0.1-50pM14fM
200GeO2Colorimetric Assay of OPsparaoxonColor0.1-50pM14fMTypically, 100 µL of different concentrations of paraoxon (0, 0.1, 2, 5, 10, 15, 30, 50, 70, 100 pm) were mixed with 20 µL of PB solutions (0.1 m, pH 8.0) containing AChE (10 µg mL−1). After the incubation for 20 min at 37 °C, 20 µL of ATCh solution (10 mm) and 20 µL of GeO2 nanozymes solution (1 mg mL−1) were added into the above mixture respectively for another 20 min incubation. The residual GeO2 nanozymes was collected by centrifugation, and added into 200 µL of acetate buffer (pH 4.0, 0.1 m) containing TMB (0.6 mm) and H2O2 (1.2 mm). Finally, the absorbance of the above reaction was measured after 30 min. Each experiment was repeated three times. The LOD was calculated by the equation LOD = (3σ/s), where σ is the standard deviation of blank signals and s is the slope of the calibration curve.