ATP induced alteration in the peroxidase-like properties of hollow Prussian blue nanocubes: a platform for alkaline phosphatase detection

References

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title
DOI
material type
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1443ATP induced alteration in the peroxidase-like properties of hollow Prussian blue nanocubes: a platform for alkaline phosphatase detectionhttps://doi.org/10.1039/d0an00405gOthersf hollow Prussian blue nanocubes (hPBNCs)

Materials

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BET
b nanozyme
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specific act
sa 10n
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1443hPBNCs80nmTEMthe cube-like hPBNCs were monodisperse with an average diameter of 80 nm

Kinetics

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T
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vmax 10n
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kcat
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kcat 10n
kcat unit
kcat/km
kcat/km 10n
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1443hPBNCsPODTMB7.0RT0.2580.016mM46.253.401-8M/s1443
1443hPBNCsPODH2O27.0RT0.9250.04mM15.131.437-8M/s1443microcal ITC

Applications

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linear range
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1443hPBNCs–ATPdetection of alkaline phosphataseAlkaline phosphatase (ALP)Color2.5-50mU/mL1.54mU/mL97.68%- 105.40%By utilizing the hPBNCs–ATP-based colorimetric assay, the recoveries of ALP in serum samples were in the range of 97.68% to 105.40% with the relative standard deviations (RSD, n = 3) from 2.13% to 3.22% (Table 2).