A nanozyme-linked immunosorbent assay based on metal–organic frameworks (MOFs) for sensitive detection of aflatoxin B1

References

ref title DOI material type comment
3514 316 A nanozyme-linked immunosorbent assay based on metal–organic frameworks (MOFs) for sensitive detection of aflatoxin B1 https://doi.org/10.1016/j.foodchem.2020.128039 MOF a MOF-linked immunosorbent assay (MOFLISA) Fe-MIL-88B

Materials

ref material size size err size unit size type size comment BET b nanozyme b 10n b unit specific act sa 10n sa unit comment
7558 316 Fe-MIL-88B 270 nm TEM MIL-88 with an average diameter of 270 nm was synthesized by the hydrothermal method 13.40 m2 ·g−1.

Kinetics

ref material enzyme type substrate pH T km km err km 10n km unit vmax vmax err vmax 10n vmax unit kcat kcat err kcat 10n kcat unit kcat/km kcat/km 10n kcat/km unit comment
6198 316 Fe-MIL-88B POD H2O2 17 mM 6.7 -8 M/s 316 316
6197 316 Fe-MIL-88B POD TMB 15 mM 4 -8 M/s 316 316

Applications

ref material application target method linear range linear ran unit LOD lod unit recovery comment
5207 316 Fe-MIL-88B we constructed an indirect competitive MOFLISA for high throughput determination of AFB1 in grain drinks AFB1 Color 0.01 to 20 ng·mL−1. 0.009 ng·mL−1 87–98% (Nestle peanut milk) 86–99%(Silk soy milk)