Histidine capped-gold nanoclusters mediated fluorescence detection of glucose and hydrogen peroxide based on glucose oxidase-mimicking property of gold nanoparticles via an …

References

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807Histidine capped-gold nanoclusters mediated fluorescence detection of glucose and hydrogen peroxide based on glucose oxidase-mimicking property of gold nanoparticles via an …https://doi.org/10.1016/j.jlumin.2020.117604Metalgold nanoparticles (AuNPs)

Materials

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807AuNPs25nmTEMThe morphology of the His-AuNCs was studied via their TEM images taken. As sit is seen from Fig. 1A, the average diameter of the synthesized His-AuNCs is about 2 nm and their morphology and size are nearly spherical and uniform. The TEM images were also utilized to estimate the average diameters of the enlarged AuNPs seeds in the presence of glucose (Fig. 1B). The average diameters of His-AuNPs seeds were 10 ± 2 nm, while the diameter of enlarged AuNPs depend on the concentration of glucose and self-catalyzed activity of AuNPs. The TEM images reveal that the AuNPs in the presence of 50 μM glucose can be enlarged to an average size of 17 nm (Fig. 1C), while the diameter of enlarged AuNPs in the presence of higher glucose concentrations of 100 μM further increased to about 25 nm (Fig. 1D).

Kinetics

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807AuNPsOXDGlucose24.6mM57.4-8M/s807

Applications

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807AuNPsdetection of hydrogen peroxideH2O2Color5-135μM3.6μM
807AuNPsdetermination of glucoseglucoseColor5-125μM3.4μM99.0-101.6%The IFE-based assay system was utilized for determining the levels of glucose in urine samples of a diabetic patients.