Ultrasensitive photoelectrochemical detection of microRNA on paper by combining a cascade nanozyme-engineered biocatalytic precipitation reaction and target-triggerable DNA motor

References

ref title DOI material type comment
3434 203 Ultrasensitive photoelectrochemical detection of microRNA on paper by combining a cascade nanozyme-engineered biocatalytic precipitation reaction and target-triggerable DNA motor https://doi.org/10.1021/acssensors.0c00632 Composite Au nanoparticles (NPs) modified by cyclodextrin (Au@CD)

Materials

ref material size size err size unit size type size comment BET b nanozyme b 10n b unit specific act sa 10n sa unit comment
7489 203 Au nanoparticles (NPs) modified by cyclodextrin (Au@CD) 20 nm As shown in Figure 2A, the Au@CD NPs showed good dispersity with a diameter around 20 nm.

Kinetics

ref material enzyme type substrate pH T km km err km 10n km unit vmax vmax err vmax 10n vmax unit kcat kcat err kcat 10n kcat unit kcat/km kcat/km 10n kcat/km unit comment
6103 203 Au@CD NPs POD H2O2 100.01 mM 203 203
6102 203 the mixture of Au@CD and CeO2 POD H2O2 800.34 mM 203 As shown in Figure 4A–C, when H2O2 was used as the substrate, the Km value of Au@CD, CeO2, and the mixture of Au@CD and CeO2 were 100.01, 20.13, and 800.34 mM, respectively.
6101 203 CeO2 POD H2O2 20.13 mM 203 As shown in Figure 4A–C, when H2O2 was used as the substrate, the Km value of Au@CD, CeO2, and the mixture of Au@CD and CeO2 were 100.01, 20.13, and 800.34 mM, respectively.
6100 203 Au@CD NPs OXD DAB 42 mM 203 the GOx-like activity of Au@CD was first investigated by using DAB as the chromogenic substrates and an absorbance at 460 nm as the quantitative referenced wavelength. As shown in Figure S5, the Michaelis–Menten constant (Km) for the Au@CD NPs was 42 mM, which was distinctly higher than that of glucose oxidase (about 4.87 mM).

Applications

ref material application target method linear range linear ran unit LOD lod unit recovery comment
5116 203 paper-based sensor MiRNA Detection. miRNA-141 E-chem 0.002-170 pM 0.6 fM 97.0–110.0% the recoveries and RSD were in the range of 97.0–110.0 and 1.31–13.64%, suggesting a gratifying analysis capability of the proposed sensor for miRNA-141 in complex clinical samples.
5115 203 paper-based sensor MiRNA Detection. miRNA-141 E-chem 0.002-170 pM 0.6 fM 97.0–110.0%