One-step fabrication of trimetallic alloy nanozyme catalyzer for luminol-H2O2 chemiluminescence and its application for miRNA-21 detection coupled with miRNA walking machine

References

ref title DOI material type comment
3636 448 One-step fabrication of trimetallic alloy nanozyme catalyzer for luminol-H2O2 chemiluminescence and its application for miRNA-21 detection coupled with miRNA walking machine https://doi.org/10.1016/j.jpba.2020.113280 PtCuCo trimetallic alloys (PtCuCo-TAs)

Materials

ref material size size err size unit size type size comment BET b nanozyme b 10n b unit specific act sa 10n sa unit comment
7685 448 PtCuCo-TAs nm SEM Note that the obtained pure Pt nanoparticles had 3D network-like porous nanostructures, as shown in Fig. 1A. And the diameters of Pt nanoparticles were ranging from 8 to 12 nm. Compared with the 3D network-like porous nanostructures of Pt, the pure Cu nanomaterials were rough and composed of many 3D irregular spherical nanoparticles with porous structures and the diameters of Cu nanomaterials were about 45 ± 3 nm as shown in Fig. 1B. Furthermore, Fig. 1C exhibited the morphology of Co nanomaterials, and it was found that Co nanomaterials displayed uniform flower-like nanostructures with diameter up to 160 ± 10 nm. Simultaneously, the images of Fig. 1D revealed that PtCuCo-TAs had irregular bulk structure. The results of morphology for different samples revealed that PtCuCo-TAs was synthesized successfully.

Kinetics

ref material enzyme type substrate pH T km km err km 10n km unit vmax vmax err vmax 10n vmax unit kcat kcat err kcat 10n kcat unit kcat/km kcat/km 10n kcat/km unit comment

Applications

ref material application target method linear range linear ran unit LOD lod unit recovery comment
5363 448 PtCuCo-TAs miRNA-21 detection miRNA-21 CL 5 × 10−16 - 1 × 10-10 M 1.67 × 10−16 M 99.0–104.4%
5362 448 PtCuCo-TAs miRNA-21 detection miRNA-21 CL 5 × 10−16 - 1 × 10-10 M 1.67 × 10−16 M 99.0–104.4% this method can effectively apply for the detection of miRNA-21 with accuracy and reliability in sample analysis.