Nanozyme Database

	ref	title	DOI	material type	comment
4408	1223	Cobalt ferrite nanozyme for efficient symbiotic nitrogen fixation via regulating reactive oxygen metabolism	https://doi.org/10.1039/D0EN00935K	Metal oxide	an antioxidant cobalt ferrite (CoFe2O4) nanozyme as a bridge between nanotechnology and biological nitrogen fixation, which was shown to efficiently regulate the reactive oxygen metabolism and protect nitrogenase

	ref	material	size	size err	size unit	size type	size comment	BET	b _nanozyme	b 10ⁿ	b unit	specific act	sa 10ⁿ	sa unit	comment
8248	1223	CoFe2O4

	ref	material	enzyme type	substrate	pH	T	k_m	k_m err	k_m 10ⁿ	k_m unit	v_max	v_max err	v_max 10ⁿ	v_max unit	k_cat	k_cat err	k_cat 10ⁿ	k_cat unit	k_cat/k_m	k_cat/k_m 10ⁿ	k_cat/k_m unit	comment
7162	1223	CoFe2O4	POD	H2O2			6.55	0.441		μM	0.0226			μM/min					1223

	ref	material	application	target	method	linear range	linear ran unit	LOD	lod unit	recovery	comment
6101	1223	CoFe2O4	an antioxidant cobalt ferrite (CoFe2O4) nanozyme as a bridge between nanotechnology and biological nitrogen fixation, which was shown to efficiently regulate the reactive oxygen metabolism and protect nitrogenase, thereby significantly enhancing the symbiotic nitrogen fixation efficiency by 260% in Glycine max (L.) Merr. (soybean). The CoFe2O4 nanozyme was also revealed to effectively reduce the concentration of ROS in the nodule by 56.6%, creating a superior environment for the proliferation of rhizobia and forming more effective nodules (larger nodules for an increase of 45.6% in the number of parasitic rhizobia). Furthermore, the CoFe2O4 nanozyme was shown to act as a synergist of leghemoglobin and increase its accumulation by 45.9%, where the high concentration of leghemoglobin in nodular cells can create a relatively hypoxic environment and protect nitrogenase, thus achieving a quantitative leap in nitrogen fixation capacity and simultaneously increasing the soybean photosynthesis by 67.2%. Our study has demonstrated that the CoFe2O4 nanozyme can efficiently regulate the intracellular ROS metabolism and serve as a promising strategy for enhancing symbiotic nitrogen fixation.	ROS