Construction of a chiral artificial enzyme used for enantioselective catalysis in live cells

References

ref title DOI material type comment
4574 1418 Construction of a chiral artificial enzyme used for enantioselective catalysis in live cells https://doi.org/10.1039/d0sc03082a Composite yolk– shell Fe3O4@Poly(L-/D-Trp)

Materials

ref material size size err size unit size type size comment BET b nanozyme b 10n b unit specific act sa 10n sa unit comment
8427 1418 Polymer shell 8.5 nm DLS Thickness
8426 1418 SiO2 shell 25 nm TEM Thickness
8425 1418 Fe3O4 NPs 200 nm TEM average diameter

Kinetics

ref material enzyme type substrate pH T km km err km 10n km unit vmax vmax err vmax 10n vmax unit kcat kcat err kcat 10n kcat unit kcat/km kcat/km 10n kcat/km unit comment
7337 1418 Fe3O4@Poly(L-Trp) POD L-tyrosinol 6.04 0.82 mM 14.8 0.85 3 1/s 1418 -6 M-1 s-1
7336 1418 Fe3O4@Poly(L-Trp) POD D-tyrosinol 14.08 0.96 mM 8.64 0.43 3 1/s 1418 -6 M-1 s-1
7335 1418 Fe3O4@Poly(D-Trp) POD L-tyrosinol 22.73 2.46 mM 10.78 1.27 3 1/s 1418 -6 M-1 s-1
7334 1418 Fe3O4@Poly(D-Trp) POD D-tyrosinol 6.49 0.86 mM 16.44 0.4 3 1/s 1418 -6 M-1 s-1

Applications

ref material application target method linear range linear ran unit LOD lod unit recovery comment
6268 1418 Fe3O4@Poly(L-/D-Trp) Nanozymes were used to selectively label live yeast cells among yeast, S. aureus, E. coli and B. subtilis bacterial cells