ATP induced alteration in the peroxidase-like properties of hollow Prussian blue nanocubes: a platform for alkaline phosphatase detection

References

ref title DOI material type comment
4594 1443 ATP induced alteration in the peroxidase-like properties of hollow Prussian blue nanocubes: a platform for alkaline phosphatase detection https://doi.org/10.1039/d0an00405g Others f hollow Prussian blue nanocubes (hPBNCs)

Materials

ref material size size err size unit size type size comment BET b nanozyme b 10n b unit specific act sa 10n sa unit comment
8448 1443 hPBNCs 80 nm TEM the cube-like hPBNCs were monodisperse with an average diameter of 80 nm

Kinetics

ref material enzyme type substrate pH T km km err km 10n km unit vmax vmax err vmax 10n vmax unit kcat kcat err kcat 10n kcat unit kcat/km kcat/km 10n kcat/km unit comment
7363 1443 hPBNCs POD TMB 7.0 RT 0.258 0.016 mM 46.25 3.401 -8 M/s 1443
7362 1443 hPBNCs POD H2O2 7.0 RT 0.925 0.04 mM 15.13 1.437 -8 M/s 1443 microcal ITC

Applications

ref material application target method linear range linear ran unit LOD lod unit recovery comment
6294 1443 hPBNCs–ATP detection of alkaline phosphatase Alkaline phosphatase (ALP) Color 2.5-50 mU/mL 1.54 mU/mL 97.68%- 105.40% By utilizing the hPBNCs–ATP-based colorimetric assay, the recoveries of ALP in serum samples were in the range of 97.68% to 105.40% with the relative standard deviations (RSD, n = 3) from 2.13% to 3.22% (Table 2).